Cooper, S.R., J. Huvane, P. Vaithiyanathan, and C.J. Richardson. 1999. Calibration of diatoms along a nutrient gradient in Florida Everglades Water Conservation Area-2A, USA. Journal of Paleolimnology 22:413-437.

Abstract. The relationship between diatom taxa preserved in surface soils and environmental variables at 31 sites in Water Conservation Area 2A (WCA-2A) of the Florida Everglades was explored using multivariate analyses.  Surface soils were collected along a phosphorus (P) gradient and analyzed for diatoms, total P, % nitrogen (N), % carbon (C), calcium (Ca) and biogenic silica (BSi).  Phosphorus varied from 315-1781 mg g-1 , and was not found to be correlated with the other geochemical variables.  Canonical correspondence analysis (CCA) was used to examine which environmental variables correlated most closely with the distributions in diatom taxa.  Canonical correspondence analysis with forward selection, constrained and partial CCA, and Monte Carlo permutation tests of significance show the most significant changes in diatom assemblages along the P gradient (p < 0.01), with additional species differences correlated with soil C, N, Ca, and BSi. 

Weighted-averaging (WA) regression and calibration models of diatom assemblages to P and BSi. were developed.  The diatom-based inference model for soil [P] had a high apparent  r2 (0.86) with RMSEboot =218 mg g-1.   Indicator diatom species identified by assessing species WA optima and WA tolerance to [P], such as Nitzschia amphibia and N. palea for high [P] (~1300-1400 mg g-1) and Achnanthes minutissima var. scotica and Mastigloia smithii for low (~400-600 mg g-1), may be useful as monitoring tools for eutrophication in WCA-2A as well as other areas of the Everglades.  Diatom assemblages analyzed by cluster analysis were related to location within WCA-2A, and dominant taxa within clusters are discussed in relation to the geochemical variables measured as well as hydrology and pH.  Diversity of diatom assemblages and a "Disturbance Index" based on diatom data are discussed in relation to the historically P-limited Everglades ecosystem.  Diatom assemblages should be very useful for reconstructions of [P] through time in the Florida Everglades, provided diatoms are well preserved in soil cores.

Key words: diatoms, Everglades, phosphorus, wetland, calibration, multivariate, Florida

Reproduced with kind permission from Kluwer Academic Publishers

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